Conjugation
Part:BBa_K5170005:Design
Designed by: Subhanan Banerjee Group: iGEM24_IISc-Bengaluru (2024-10-01)
A novel PROTAC using BBa_K5170003 against 231pTau
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
C8 Alkyne dCTP is a modified base having a alkyne label and [S,R,S]-AHPC is a ligand for VHL E3 Ligase. These were bought from Sigma Aldrich. The Aptamer sequence were obtained from literature, available at BBa K5170003.
Source
The aptamer was selected by SELEX; randomisation of a library followed by negative and positive binding selection rounds in the original literature.
References
Teng, I-Ting., Li, X., Yadikar, H. A., Yang, Z., Li, L., Lyu, Y., Pan, X., Wang, K. K., & Tan, W. (2018). Identification and Characterization of DNA Aptamers Specific for Phosphorylation Epitopes of Tau Protein. Journal of the American Chemical Society, 140(43), 14314–14323. https://doi.org/10.1021/jacs.8b08645.