Conjugation

Part:BBa_K5170005:Design

Designed by: Subhanan Banerjee   Group: iGEM24_IISc-Bengaluru   (2024-10-01)


A novel PROTAC using BBa_K5170003 against 231pTau


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

C8 Alkyne dCTP is a modified base having a alkyne label and [S,R,S]-AHPC is a ligand for VHL E3 Ligase. These were bought from Sigma Aldrich. The Aptamer sequence were obtained from literature, available at BBa K5170003.

Source

The aptamer was selected by SELEX; randomisation of a library followed by negative and positive binding selection rounds in the original literature.

References

Teng, I-Ting., Li, X., Yadikar, H. A., Yang, Z., Li, L., Lyu, Y., Pan, X., Wang, K. K., & Tan, W. (2018). Identification and Characterization of DNA Aptamers Specific for Phosphorylation Epitopes of Tau Protein. Journal of the American Chemical Society, 140(43), 14314–14323. https://doi.org/10.1021/jacs.8b08645.